本公司产品仅供科研使用,不用于人体及临床诊断

小鼠睾酮(T)ELISA检测试剂盒

英文名称: Mouse Testosterone (T) ELISA Kit
实验类型: 竞争法
检测范围: 0.156-10ng/mL
灵敏度: 0.067ng/mL
种属: 小鼠
保存温度: 2-8℃
样本类型: 血清、血浆
产品说明书
品牌 货号 纯度/规格 包装 市场价 活动价格 库存 数量 购物车
江莱生物 JL25196-48T 48T 1盒 ¥1800.00
江莱生物 JL25196-5×96T 5×96T 1盒 ¥9775.00
江莱生物 JL25196-96T 96T 1盒 ¥2300.00

|   别称

 

T; Testesteron; Testeronun

 

 

|   T 简介

 

睾丸素,又称睾酮、睾丸酮、睾甾酮,分子式为 C19H28O2。睾酮是一种雄性激素类固醇,在第三和第十七位分别含有一个酮基和一个羟基。它通过几个步骤从胆固醇中生物合成,并在肝脏中转化为非活性代谢物。睾酮通过与雄性激素受体结合并激活雄性激素受体而发挥其作用。在大多数脊椎动物中, 睾酮主要由男性的睾丸分泌,在较小程度上由女性的卵巢分泌。

 

 

|   特异性

 

可检测样本中小鼠的T,且与其类似物无明显交叉反应

 

 

|   典型数据及参考曲线

 

由于实验操作条件的不同( 如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

 

浓度(ng/mL) 10 5 2.5 1.25 0.625 0.312 0.156 0
OD值 0.216 0.298 0.417 0.669 0.955 1.405 1.79 1.865

 

 

|   重复性

 

板内变异系数小于10%,板间变异系数小于10%

 

 

|   回收率

 

在选取的健康小鼠血清、血浆中加入3个不同浓度水平的小鼠T,计算回收率

 

样本类型 范围 平均回收率
血清(n=8) 81-101 96
血浆(n=8) 92-105 101

 

 

|   线性稀释

 

分别在选取的4份健康小鼠血清、血浆中加入高浓度小鼠T,在标准曲线动力学范围内进行稀释,评估线性。

 

稀释比例 回收率(%) 血清 血浆
1:2 范围(%) 83-95 88-96
平均回收率(%) 91 93
1:4 范围(%) 89-104 87-108
平均回收率(%) 93 98

 

 

注:ELISA试剂盒检测范围等数据,因检测样本 的不同而调整,实际数据以随货说明书为准。

 

|   客户发表的文献

 

 

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DOI: 10.1002/adma.202310673

 


 

The circular RNA circINPP4B acts as a sponge of miR-30a to regulate Th17 cell differentiation during progression of experimental autoimmune encephalomyelitis

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Journal: Cellular & Molecular Immunology

Year: 2021

DOI: 10.1038/s41423-021-00748-y

 


 

Parabacteroides distasonis uses dietary inulin to suppress NASH via its metabolite pentadecanoic acid

Rank: IF 20.5

Journal: Nature Microbiology

Year: 2023

DOI: 10.1038/s41564-023-01418-7

 


 

An emerging antibacterial nanovaccine for enhanced chemotherapy by selec‐tively eliminating tumor-colonizing bacteria

Rank: IF 18.8

Journal: Science Bulletin

Year: 2024

DOI: 10.1016/j.scib.2024.06.016

 


 

A Polysaccharide Metal Phenolic Network Nanocomposite for Theranostic Applications in Inflammatory Bowel Disease

Rank: IF 18.5

Journal: Advanced Functional Materials

Year: 2024

DOI: 10.1002/adfm.202416918

 


 

Oral Nano-antioxidants Improve Sleep by Restoring Intestinal Barrier Integrity and Preventing Systemic Inflammation

Rank: IF 16.3

Journal: National Science Review

Year: 2023

DOI: 10.1093/nsr/nwad309

 


 

Gastrointestinal MicroenvironmentResponsive Nanoencapsulation of Probioticsand Drugs for Synergistic Therapy ofIntestinal Diseases

Rank: IF 15.8

Journal: ACS Nano

Year: 2023

DOI: 10.1021/acsnano.3c02646

 


 

Neutrophil-Mimetic Upconversion Photosynthetic Nanosystem Derived from Microalgae for Targeted Treatment of Thromboembolic Stroke

Rank: IF 15.8

Journal: ACS Nano

Year: 2024

DOI: 10.1021/acsnano.4c06247

 


 

Novel compound FLZ alleviates rotenone-induced PD mouse model by suppressing TLR4/MyD88/NF-κB pathway through microbiota–gut–brain axis

Rank: IF 14.7

Journal: Acta Pharmaceutica Sinica B

Year: 2021

DOI: 10.1016/j.apsb.2021.03.020

 


 

Enrichment and sensing tumor cells by embedded immunomodulatory DNA hydrogel to inhibit postoperative tumor recurrence

Rank: IF 14.7

Journal: Nature Communications

Year: 2023

DOI: 10.1038/s41467-023-40085-4