cCAT; AAT; ASAT; SGOT; GOT1; Cysteine transaminase, cytoplasmic; Transaminase A; Aspartate Transaminase 1,Cytoplasmic; Glutamic-Oxaloacetic Transaminase 1,Soluble
天门冬氨酸氨基转移酶(AST)或天门冬氨酸转氨酶,又称AspAT/ASAT/AAT或(血清)谷氨酸草酰乙酸转氨酶(GOT,SGOT),是一种依赖磷酸吡哆醛(PLP)的转氨酶(AST催化天门冬氨酸和谷氨酸之间α-氨基的可逆转移,因此它是氨基酸代谢中的一个重要酶。AST存在于肝脏、心脏、骨骼肌、肾脏、大脑和红血球中。
可检测样本中小鼠的AST,且与其类似物无明显交叉反应。
由于实验操作条件的不同( 如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。
注意:本图仅供参考,应以同次试验标准品所绘标准曲线计算标本含量。
板内变异系数小于10%,板间变异系数小于10%
在选取的健康小鼠血清、血浆、细胞培养上清中加入3个不同浓度水平的小鼠AST,计算回收率
分别在选取的4份健康小鼠血清、血浆、细胞培养上清中加入高浓度小鼠AST,在标准曲线动力学范围内进行稀释,评估线性。
注:ELISA试剂盒检测范围等数据,因检测样本 的不同而调整,实际数据以随货说明书为准。
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