Lactate Dehydrogenase
乳酸脱氢酶(LDH或LD)是一个广泛存在于各种生物体中的酶,包括植物与动物。它存在于四种不同的酶分类中,其中两种是依赖细胞色素c的酶,它们各自作用于D-乳酸与L-乳酸。LDH存在于身体的许多组织和器官中,包括肌肉、肝脏、心脏、胰腺、肾脏、大脑和血细胞。
可检测样本中大鼠的LDH,且与其类似物无明显交叉反应。
由于实验操作条件的不同( 如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。
注意:本图仅供参考,应以同次试验标准品所绘标准曲线计算标本含量。
板内变异系数小于10%,板间变异系数小于10%
在选取的健康大鼠血清、血浆中加入3个不同浓度水平的大鼠LDH,计算回收率
分别在选取的4份健康大鼠血清、血浆中加入高浓度大鼠LDH,在标准曲线动力学范围内进行稀释,评估线性。
注:ELISA试剂盒检测范围等数据,因检测样本 的不同而调整,实际数据以随货说明书为准。
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