本公司产品仅供科研使用,不用于人体及临床诊断

鸡肿瘤坏死因子α(TNF-α)ELISA检测试剂盒

英文名称: Chicken Tumor Necrosis Factor Alpha (TNFa) ELISA Kit
实验类型: 双抗体夹心法
检测范围: 3.12-200pg/mL(特殊要求可定制)
灵敏度: 1.4pg/mL
种属: 鸡
保存温度: 2-8℃
样本类型: 组织匀浆、细胞裂解液、细胞培养上清 和 其他生物液体
产品说明书
品牌 货号 纯度/规格 包装 市场价 会员价格 库存 数量 购物车
江莱生物 JL21706-48T 48T 1盒 ¥1680.00
江莱生物 JL21706-96T 96T 1盒 ¥2160.00

|   别称

 

DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Lig和 Superfamily Member 2

 

 

|   TNF-α 简介

 

肿瘤坏死因子α(TNF-α)由中性粒细胞、活化的淋巴细胞、巨噬细胞、NK细胞、LAK细胞、星形胶质细胞内皮细胞、平滑肌细胞和一些转化细胞产生。自然发生的TNF-α形式是糖基化的,但非糖基化的重组TNF-α具有相应的生物活性。

 

 

|   特异性

 

可检测样本中鸡的TNF-α,且与其类似物无明显交叉反应

 

 

|   典型数据及参考曲线

 

由于实验操作条件的不同( 如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

 

浓度(pg/mL) 200 100 50 25 12.5 6.25 3.12 0
OD值 2.33 1.41 0.78 0.49 0.32 0.25 0.2 0.09
校正OD值 2.24 1.32 0.69 0.4 0.23 0.16 0.11 -

 

注意:本图仅供参考,应以同次试验标准品所绘标准曲线计算标本含量。

 

 

|   重复性

 

板内变异系数小于10%,板间变异系数小于10%

 

 

|   回收率

 

在选取的健康鸡血清、血浆、细胞培养上清中加入3个不同浓度水平的鸡TNF-α,计算回收率

 

样本类型 范围 平均回收率
血清(n=8) 84-101 96
血浆(n=8) 92-105 102
细胞培养上清(n=8) 96-108 105

 

 

|   线性稀释

 

分别在选取的4份健康鸡血清、血浆、细胞培养上清中加入高浓度鸡TNF-α,在标准曲线动力学范围内进行稀释,评估线性。

 

稀释比例 回收率(%) 血清 血浆 细胞培养上清
1:2 范围(%) 84-95 88-96 89-110
平均回收率(%) 91 93 96
1:4 范围(%) 89-103 87-108 105-115
平均回收率(%) 94 98 108

 

 

注:ELISA试剂盒检测范围等数据,因检测样本 的不同而调整,实际数据以随货说明书为准。

 

 

|   ELISA操作视频

 

ELISA操作视频        ELISA保存温度

 

 

|   ELISA操作前必读 / 下载

 

样本处理    血液样本    加样    温育

 

洗板    拟合曲线    常见问题    下载

 

 

|   客户发表的文献

 

 

Rutin ameliorates gout via reducing XOD activity, inhibiting ROS production and NLRP3 inflammasome activation in quail

Rank: IF 7.5

Journal: Biomedicine & Pharmacotherapy

Year: 2022

DOI: 10.1016/j.biopha.2022.114175

 


 

Overnutrition-induced gout: An immune response to NLRP3 inflammasome dysregulation by XOD activity increased in quail

Rank: IF 7.3

Journal: Frontiers in Immunology

Year: 2022

DOI: 10.3389/fimmu.2022.1074867

 


 

Ammonia exposure induced intestinal inflammation injury mediated by intestinal microbiota in broiler chickens via TLR4/TNF-α signaling pathway.

Rank: IF 6.8

Journal: Ecotoxicology and Environmental Safety

Year: 2021

DOI: 10.1016/j.ecoenv.2021.112832

 


 

Gut microbiota dysbiosis exaggerates ammonia-induced tracheal injury Via TLR4 signaling pathway.

Rank: IF 6.8

Journal: Ecotoxicology and Environmental Safety

Year: 2022

DOI: 10.1016/j.ecoenv.2022.114206

 


 

Restorative effects of Lactobacillus rhamnosus LR-32 on the gut microbiota, barrier integrity, and 5-HT metabolism in reducing feather-pecking behavior in laying hens with antibiotic-induced dysbiosis

Rank: IF 5.2

Journal: Frontiers in Microbiology

Year: 2023

DOI: 10.3389/fmicb.2023.1173804

 


 

Malfunctioned inflammatory response and serotonin metabolism at the microbiota-gut-brain axis drive feather pecking behavior in laying hens: Animal Well-Being and Behavior

Rank: IF 4.4

Journal: Poultry Science

Year: 2023

DOI: 10.1016/j.psj.2023.102686

 


 

Preparation and sulfate modified of Lagenaria siceraria (Molina) Standl polysaccharide and its immune-enhancing adjuvant activity

Rank: IF 4.4

Journal: Poultry Science

Year: 2022

DOI: 10.1016/j.psj.2022.102112

 


 

Lactobacillus casei DBN023 protects against jejunal mucosal injury in chicks infected with Salmonella pullorum CMCC-533

Rank: IF 2.4

Journal: Research in Veterinary Science

Year: 2019

DOI: 10.1016/j.rvsc.2019.09.010

 


 

Dietary Lactobacillus casei can be used to influence intraepithelial lymphocyte migration and modulate mucosal immunity in chicks

Rank: IF 2

Journal: British Poultry Science

Year: 2021

DOI: 10.1080/00071668.2021.1889464

 


 

Resveratrol Attenuates LPS-induced Apoptosis via Inhibiting NF-κ B Activity in Chicken Peripheral Lymphocyte Cultures

Rank: IF 1.1

Journal: Brazilian Journal of Poultry Science

Year: 2018

DOI: 10.1590/1806-9061-2017-0720