探讨激活素A对小鼠腹腔巨噬细胞活性的调节作用及其机制.方法:免疫组化观察激活素A(ACV-A)在巨噬细胞上的表达,ELISA法检测小鼠腹腔巨噬细胞培养上清中ACV-A分泌水平,采用RT-PCR检测小鼠腹腔巨噬细胞ACV-A mRNA、ActRⅡA mRNA及激活素受体相互作用蛋白2(ActRIP2) mRNA的表达.结果:激活素A可以促进原代培养小鼠腹腔巨噬细胞分泌ACV-A及ACV-A mRNA表达,并呈剂量依赖关系。

  胎膜早破(PROM)是危害母婴健康的产科常见病，发病率约占分娩总数的3.03％—21.9％。胎膜破裂后，由于屏障作用消失，将引起:羊膜腔感染、早产、围产期感染、败血症、新生儿窒息等严重的并发症，因此胎膜早破一直受到产科界的关注。本实验采用ELISA的方法，分别测定胎膜早破孕妇及正常晚期妊娠妇女血浆、羊水中MMP-9水平和羊水中白介素6(IL-6)水平，及在胎膜早破患者羊水中MMP-9和白介素6(IL-6)相关性的研究。

  In order to measure the concentration of  in the sample, this  ELISA Kit includes a set of calibration standards.The concentration of  in the samples is then determined by comparing the O.D. of the samples to the standard curve.

   The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus GATA-3 concentration. 

  The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus E-Selectin concentration. 

  The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus E-Cad concentration.

  CD105 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus ENG;CD105 concentration.

  The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus D-LDH concentration. 

  The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus D-LA concentration.

  The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus D-xylose concentration.