CORT
皮质酮,又称 17-脱氧皮质醇和 11β,21-二羟基黄体酮,是一种 21 碳的皮质 类固醇激素,在肾上腺皮质中产生。除了 17α-羟化酶缺乏导致的非常罕见的 先天性肾上腺增生症外,它对人类的重要性不大。在许多物种中,包括两栖 动物、爬行动物、啮齿动物和鸟类,皮质酮是一种主要的糖皮质激素,参与 调节能量、免疫反应和压力反应。皮质酮通过醛固酮合成酶转化为醛固酮, 醛固酮合成酶仅在肾小球细胞的线粒体中发现。肾小球细胞存在于肾小球区, 它是肾上腺皮质内分泌细胞的最表层区域。皮质酮是矿物皮质激素醛固酮的 前体分子,是体内钠和钾水平的主要平衡调节剂之一。
可检测样本中小鼠的CORT,且与其类似物无明显交叉反应。
由于实验操作条件的不同( 如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。
板内变异系数小于10%,板间变异系数小于10%
在选取的健康小鼠血清、血浆中加入3个不同浓度水平的小鼠CORT,计算回收率
分别在选取的4份健康小鼠血清、血浆中加入高浓度小鼠CORT,在标准曲线动力学范围内进行稀释,评估线性。
注:ELISA试剂盒检测范围等数据,因检测样本 的不同而调整,实际数据以随货说明书为准。
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