E2
雌二醇(Estradiol,E2),亦称“动情素”、“求偶素”。化学式 C18H24O2, 分子量为 272.38,有α,β两种类型,雌激素的一种。含量最多,活性也最强。由卵巢内卵泡的颗粒细胞分泌,其代谢物是雌酮及雌三醇。含 18 个碳原子。其靶器官为子宫、阴道、输卵管和垂体。可作为一种为经皮肤吸收的 雌激素治疗剂。雌激素能促使细胞合成 DNA、RNA 和相应组织内各种不同的蛋白质。
可检测样本中小鼠的E2,且与其类似物无明显交叉反应。
由于实验操作条件的不同( 如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。
板内变异系数小于10%,板间变异系数小于10%
在选取的健康小鼠血清、血浆和细胞培养上清中加入3个不同浓度水平的小鼠E2,计算回收率
分别在选取的4份健康小鼠血清、血浆和细胞培养上清中加入高浓度小鼠E2,在标准曲线动力学范围内进行稀释,评估线性。
注:ELISA试剂盒检测范围等数据,因检测样本 的不同而调整,实际数据以随货说明书为准。
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