Follicle Stimulating Hormone
卵泡刺激素(FSH)是一种促性腺激素,也是一种糖蛋白多肽激素。FSH由垂体前叶的促性腺激素细胞合成和分泌,它和黄体生成素(LH)在生殖系统中共同发挥作用。FSH是一个35.5 kDa的糖蛋白异构体,由两个多肽单元组成,即α和β。其结构与黄体生成素(LH)、促甲状腺激素(TSH)和人绒毛膜促性腺激素(hCG)的结构相似。LH、FSH、TSH和hCG糖蛋白的α亚单位是相同的,由96个氨基酸组成,而β亚单位则不同。这两个亚单位都是生物活性所必需的。FSH有一个111个氨基酸的β亚单位(FSH β),赋予其特定的生物作用,并负责与卵泡刺激素受体相互作用。激素的糖类部分与天冬酰胺共价键合,由N-乙酰半乳糖胺、甘露糖、N-乙酰葡萄糖胺、半乳糖和唾液酸组成。
可检测样本中大鼠的FSH,且与其类似物无明显交叉反应。
由于实验操作条件的不同( 如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。
板内变异系数小于10%,板间变异系数小于10%
在选取的健康大鼠血清、血浆中加入3个不同浓度水平的大鼠FSH,计算回收率
分别在选取的4份健康大鼠血清、血浆中加入高浓度大鼠FSH,在标准曲线动力学范围内进行稀释,评估线性。
注:ELISA试剂盒检测范围等数据,因检测样本 的不同而调整,实际数据以随货说明书为准。
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